At the beginning, the extracellular matrix is synthesized in order to repair the epidermal barrier [ 6 ]. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats.
Asano, S. In all groups, the wounds were cleaned with saline 0. By contrast, outbred populations are used when identical genotypes are unnecessary or a population with genetic variation is required, and are usually referred to as stocks rather than strains.
Blood was collected from the heart and taken into tubes with EDTA as anticoagulant.
Abstract The wound-healing process is complex and remains a challenging process under the influence of several factors, including eating habits. Key words humanized rat model, impaired glucose tolerance, prediabetes, high fat diet, Glut4 mRNA, insulin immunohistochemistry Introduction Diabetes is a chronic disease and for the most part a silent hidden disease.
Carbonyl protein levels were determined by the method adapted from Jana et al. As observed here, increased cell number was also shown in the skin from animals fed a diet rich in trans fats [ 3637 ] and in the stomach from rats that received high doses of alcohol [ 38 ], showing that the consumption of these items can lead to inflammatory infiltrate tissue and delay the healing process.
Erythrocyte antioxidant enzyme activities Blood was treated to obtain the erythrocyte lysates as specified in the kits before assaying for SOD and GPx activities. Supernatant was collected for reading. Vijayaraghavan, and P. In our study, the excess production of reactive species of thiobarbituric acid malondialdehyde and protein carbonyls showed a negative effect of combining a high-fat diet and alcohol on skin repair.
Chemical mediators are known to participate actively in wound healing during all three stages [ 2 ]. Fibroblasts are the main cells involved with collagen III-rich granulation tissue, which is gradually replaced by the more resistant collagen I [ 78 ].
Tissue samples in each day were obtained from different wounds at days 7, 14, and 21 for histological, biochemical, and cytokine expression analysis. PLoS One 9: Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet.
Introduction Skin wound healing is an essential dynamic process, which includes basically three stages: Typically, when markers of oxidative lesions such as MDA and CP are elevated, antioxidant defense systems are also activated to neutralize free radicals, resulting in decreased markers of oxidative stress in tissues.
An increase in stress markers may lead to chronic inflammation and cell death, contributing to tissuemorphological alterations and the formation of a fragile scar tissue. To make sure about the specificity of amplified products, melting curve for each individual sample was obtained at the end of amplification.
Shaking rat Kawasaki was first described in To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study.
Endothelium-dependent relaxation analyses in mesenteric microvessels. Control diet, Husain, E. The rats were being raised for laboratory use by Dr. Table 2: Figure 7. Blood Marker Tests Blood was collected during euthanasia, after 12 h of fasting, through cardiac puncture using a heparinized syringe.Animals and experimental design: Male Wistar rats, weighing gm were employed in the present study and were fed on standard chow diet or high fat diet.
Food and water were provided ad libitum throughout experimental period. High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar RatsAuthor: Daiane Figueiredo Rosa, Mariáurea Matias Sarandy, Rômulo Dias Novaes, Mariella Bontempo Freitas, Mar.
· High-cholesterol diet enriched with onion affects endothelium-dependent relaxation and NADPH oxidase activity in mesenteric microvessels from Wistar rats.
· The kidney function status of high fat diet-streptozotocin (HFD-STZ) induced NIDDM in albino rats fed antioxidant supplementation was monitored in vsfmorocco.com: Chinaka Nc, Chuku Lc, Monago-Ighorodje Cc, Edeogu O.
Four (4) months HFD-feeding to young Wistar rats to developed insulin resistance and 6 months HFD feeding developed pre-diabetes in young Wistar rats.
Progressive changes in glucose uptake and GLUT-4 gene expression were observed in this prediabetes model rats. Are Sprague Dawley rats better than Wistar rats for obesity (high fat diets) and metabolic syndrome investigation?